NGS Comparison
August 3rd, 2011 Posted by admin
NGS Comparison
|
Ion Torrent |
454 Sequencing |
Illumina |
SOLiD |
| Sequencing Chemistry |
Ion semiconductor sequencing |
Pyrosequencing |
Polymerase-based sequence-by-synthesis |
Ligation-based sequencing |
| Amplification approach |
Emulsion PCR |
Emulsion PCR |
Bridge amplification |
Emulsion PCR |
| Mb per run |
100 Mb |
100 Mb |
600 Gb |
3000 Mb |
| Time per run |
2 hours |
7 hours |
9 days |
5 days |
| Read length |
100 bp |
400 bp |
2×100 bp |
35-50 bp |
| Cost per run |
$ 500 USD |
$ 8,438 USD |
$ 20,000 USD |
$ 17,447 USD |
| Cost per Mb |
$ 5.00 USD |
$ 84.39 USD |
$ 0.03 USD |
$ 5.81 USD |
| Cost per instrument |
$ 50,000 USD |
$ 500,000 USD |
$ 600,000 USD |
$ 591,000 USD |
Add a Comment Trackback