NGS Comparison
| Ion Torrent | 454 Sequencing | Illumina | SOLiD | |
|---|---|---|---|---|
| Sequencing Chemistry | Ion semiconductor sequencing | Pyrosequencing | Polymerase-based sequence-by-synthesis | Ligation-based sequencing |
| Amplification approach | Emulsion PCR | Emulsion PCR | Bridge amplification | Emulsion PCR |
| Mb per run | 100 Mb | 100 Mb | 600 Gb | 3000 Mb |
| Time per run | 2 hours | 7 hours | 9 days | 5 days |
| Read length | 100 bp | 400 bp | 2×100 bp | 35-50 bp |
| Cost per run | $ 500 USD | $ 8,438 USD | $ 20,000 USD | $ 17,447 USD |
| Cost per Mb | $ 5.00 USD | $ 84.39 USD | $ 0.03 USD | $ 5.81 USD |
| Cost per instrument | $ 50,000 USD | $ 500,000 USD | $ 600,000 USD | $ 591,000 USD |
Leave a Comment