August, 2011

What is a Corporation?

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NGS Comparison

NGS Comparison

Ion Torrent 454 Sequencing Illumina SOLiD
Sequencing Chemistry Ion semiconductor sequencing Pyrosequencing Polymerase-based sequence-by-synthesis Ligation-based sequencing
Amplification approach Emulsion PCR Emulsion PCR Bridge amplification Emulsion PCR
Mb per run 100 Mb 100 Mb 600 Gb 3000 Mb
Time per run 2 hours 7 hours 9 days 5 days
Read length 100 bp 400 bp 2×100 bp 35-50 bp
Cost per run $ 500 USD $ 8,438 USD $ 20,000 USD $ 17,447 USD
Cost per Mb $ 5.00 USD $ 84.39 USD $ 0.03 USD $ 5.81 USD
Cost per instrument $ 50,000 USD $ 500,000 USD $ 600,000 USD $ 591,000 USD

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